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Lepidoptera Temporal range: Early Jurassic–present, 200–0 Ma PreЄ Є O S D C P T J K Pg N
Monarch butterfly and luna moth, two widely recognized lepidopterans
Scientific classification
Kingdom:	Animalia
Phylum:	Euarthropoda
Class:	Insecta
(unranked):	Amphiesmenoptera
Order:	Lepidoptera Linnaeus, 1758
Suborders
Aglossata Glossata Heterobathmiina Zeugloptera

Lepidoptera (/ˌlɛpɪˈdɒptərə/ LEP-i-DOP-tər-ə) is an order of insects that includes butterflies and moths (both are called lepidopterans). About 180,000 species of the Lepidoptera are described, in 126 families^[1] and 46 superfamilies,^[2] 10 per cent of the total described species of living organisms.^[2][3] It is one of the most widespread and widely recognizable insect orders in the world.^[4] The Lepidoptera show many variations of the basic body structure that have evolved to gain advantages in lifestyle and distribution. Recent estimates suggest the order may have more species than earlier thought,^[5] and is among the four most speciose orders, along with the Hymenoptera, Diptera, and Coleoptera.^[4]

Lepidopteran species are characterized by more than three derived features. The most apparent is the presence of scales that cover the bodies, wings, and a proboscis. The scales are modified, flattened "hairs", and give butterflies and moths their wide variety of colors and patterns. Almost all species have some form of membranous wings, except for a few that have reduced wings or are wingless. Mating and the laying of eggs are carried out by adults, normally near or on host plants for the larvae. Like most other insects, butterflies and moths are holometabolous, meaning they undergo complete metamorphosis. The larvae are commonly called caterpillars, and are completely different from their adult moth or butterfly forms, having a cylindrical body with a well-developed head, mandible mouth parts, three pairs of thoracic legs and from none up to five pairs of prolegs. As they grow, these larvae change in appearance, going through a series of stages called instars. Once fully matured, the larva develops into a pupa. A few butterflies and many moth species spin a silk case or cocoon prior to pupating, while others do not, instead going underground.^[4] A butterfly pupa, called a chrysalis, has a hard skin, usually with no cocoon. Once the pupa has completed its metamorphosis, a sexually mature adult emerges.

The Lepidoptera have, over millions of years, evolved a wide range of wing patterns and coloration ranging from drab moths akin to the related order Trichoptera, to the brightly colored and complex-patterned butterflies.^[1] Accordingly, this is the most recognized and popular of insect orders with many people involved in the observation, study, collection, rearing of, and commerce in these insects. A person who collects or studies this order is referred to as a lepidopterist.

Butterflies and moths play an important role in the natural ecosystem as pollinators and as food in the food chain; conversely, their larvae are considered very problematic to vegetation in agriculture, as their main source of food is often live plant matter. In many species, the female may produce from 200 to 600 eggs, while in others, the number may approach 30,000 eggs in one day. The caterpillars hatching from these eggs can cause damage to large quantities of crops. Many moth and butterfly species are of economic interest by virtue of their role as pollinators, the silk they produce, or as pest species.

Etymology

The term was coined by Linnaeus in 1735 and is derived from Greek λεπίς, gen. λεπίδος ("scale") and πτερόν ("wing").^[6] Sometimes, the term Rhopalocera is used for the clade of all butterfly species, derived from the Ancient Greek ῥόπαλον (rhopalon)^[7]:4150 and κέρας (keras)^[7]:3993 meaning "club" and "horn", respectively, coming from the shape of the antennae of butterflies.

The origins of the common names "butterfly" and "moth" are varied and often obscure. The English word butterfly is from Old English buttorfleoge, with many variations in spelling. Other than that, the origin is unknown, although it could be derived from the pale yellow color of many species' wings suggesting the color of butter.^[8][9] The species of Heterocera are commonly called moths. The origins of the English word moth are more clear, deriving from the Old English moððe" (cf. Northumbrian dialect mohðe) from Common Germanic (compare Old Norse motti, Dutch mot and German Motte all meaning "moth"). Perhaps its origins are related to Old English maða meaning "maggot" or from the root of "midge", which until the 16th century was used mostly to indicate the larva, usually in reference to devouring clothes.^[10]

The etymological origins of the word "caterpillar", the larval form of butterflies and moths, are from the early 16th century, from Middle English catirpel, catirpeller, probably an alteration of Old North French catepelose: cate, cat (from Latin cattus) + pelose, hairy (from Latin pilōsus).^[11]

Distribution and diversity

The Lepidoptera are among the most successful groups of insects. They are found on all continents, except Antarctica, and inhabit all terrestrial habitats ranging from desert to rainforest, from lowland grasslands to mountain plateaus, but almost always associated with higher plants, especially angiosperms (flowering plants).^[12] Among the most northern dwelling species of butterflies and moths is the Arctic Apollo (Parnassius arcticus), which is found in the Arctic Circle in northeastern Yakutia, at an altitude of 1500 m above sea level.^[13] In the Himalayas, various Apollo species such as Parnassius epaphus have been recorded to occur up to an altitude of 6,000 m above sea level.^[14]:221

Some lepidopteran species exhibit symbiotic, phoretic, or parasitic lifestyles, inhabiting the bodies of organisms rather than the environment. Coprophagous pyralid moth species, called sloth moths, such as Bradipodicola hahneli and Cryptoses choloepi, are unusual in that they are exclusively found inhabiting the fur of sloths, mammals found in Central and South America.^[15][16]
Two species of Tinea moths have been recorded as feeding on horny tissue and have been bred from the horns of cattle. The larva of Zenodochium coccivorella is an internal parasite of the coccid Kermes species. Many species have been recorded as breeding in natural materials or refuse such as owl pellets, bat caves, honeycombs or diseased fruit.^[16]

As of 2007, there was roughly 174,250 lepidopteran species described, with butterflies and skippers estimated to comprise around 17,950, and moths making up the rest.^[2][17] The vast majority of Lepidoptera are to be found in the tropics, but substantial diversity exists on most continents. North America has over 700 species of butterflies and over 11,000 species of moths,^[18][19] while about 400 species of butterflies and 14,000 species of moths are reported from Australia.^[20] The diversity of Lepidoptera in each faunal region has been estimated by John Heppner in 1991 based partly on actual counts from the literature, partly on the card indices in the Natural History Museum (London) and the National Museum of Natural History (Washington), and partly on estimates:^[5]

External morphology

Parts of an adult butterfly

A – head, B – thorax, C – abdomen, 1 – prothoracic shield, 2 – spiracle, 3 – true legs, 4 – midabdominal prolegs, 5 – anal proleg, 6 – anal plate, 7 – tentacle, a – frontal triangle, b – stemmata (ocelli), c – antenna, d – mandible, e – labrum.

Lepidoptera are morphologically distinguished from other orders principally by the presence of scales on the external parts of the body and appendages, especially the wings. Butterflies and moths vary in size from microlepidoptera only a few millimeters long, to conspicuous animals with a wingspan greater than 25 centimetres, such as the Queen Alexandra's birdwing and Atlas moth.^[21]:246
Lepidopterans undergo a four-stage life cycle: egg; larva or caterpillar; pupa or chrysalis; and imago (plural: imagines) / adult and show many variations of the basic body structure, which give these animals advantages for diverse lifestyles and environments.

Head

Face of a caterpillar with the mouthparts showing

The head is where many sensing organs and the mouth parts are found. Like the adult, the larva also has a toughened, or sclerotized head capsule.^[22] Here, two compound eyes, and chaetosema, raised spots or clusters of sensory bristles unique to Lepidoptera, occur, though many taxa have lost one or both of these spots. The antennae have a wide variation in form among species and even between different sexes. The antennae of butterflies are usually filiform and shaped like clubs, those of the skippers are hooked, while those of moths have flagellar segments variously enlarged or branched. Some moths have enlarged antennae or ones that are tapered and hooked at the ends.^{[23]:559–560}

The maxillary galeae are modified and form an elongated proboscis. The proboscis consists of one to five segments, usually kept coiled up under the head by small muscles when it is not being used to suck up nectar from flowers or other liquids. Some basal moths still have mandibles, or separate moving jaws, like their ancestors, and these form the family Micropterigidae.^{[22][23]:560[24]}

The larvae, called caterpillars, have a toughened head capsule. Caterpillars lack the proboscis and have separate chewing mouthparts.^[22] These mouthparts, called mandibles, are used to chew up the plant matter that the larvae eat. The lower jaw, or labium, is weak, but may carry a spinneret, an organ used to create silk. The head is made of large lateral lobes, each having an ellipse of up to six simple eyes.^{[23]:562–563}

Thorax

The thorax is made of three fused segments, the prothorax, mesothorax, and metathorax, each with a pair of legs. The first segment contains the first pair of legs. In some males of the butterfly family Nymphalidae, the forelegs are greatly reduced and are not used for walking or perching.^[23]:586 The three pairs of legs are covered with scales. Lepidoptera also have olfactory organs on their feet, which aid the butterfly in "tasting" or "smelling" out its food.^[25] In the larval form there are 3 pairs of true legs, with up to 11 pairs of abdominal legs (usually eight) and hooklets, called apical crochets.^[12]

The two pairs of wings are found on the middle and third segments, or mesothorax and metathorax, respectively. In the more recent genera, the wings of the second segment are much more pronounced, although some more primitive forms have similarly sized wings of both segments. The wings are covered in scales arranged like shingles, which form an extraordinary variety of colors and patterns. The mesothorax has more powerful muscles to propel the moth or butterfly through the air, with the wing of this segment (forewing) having a stronger vein structure.^[23]:560 The largest superfamily, the Noctuidae, has their wings modified to act as tympanal or hearing organs.^[26]

The caterpillar has an elongated, soft body that may have hair-like or other projections, three pairs of true legs, with none to 11 pairs of abdominal legs (usually eight) and hooklets, called apical crochets.^[12] The thorax usually has a pair of legs on each segment. The thorax is also lined with many spiracles on both the mesothorax and metathorax, except for a few aquatic species, which instead have a form of gills.^[23]:563

Abdomen

Caterpillar prolegs on Papilio machaon

The abdomen, which is less sclerotized than the thorax, consists of 10 segments with membranes in between, allowing for articulated movement. The sternum, on the first segment, is small in some families and is completely absent in others. The last two or three segments form the external parts of the species' sex organs. The genitalia of Lepidoptera are highly varied and are often the only means of differentiating between species. Male genitals include a valva, which is usually large, as it is used to grasp the female during mating. Female genitalia include three distinct sections.

The females of basal moths have only one sex organ, which is used for copulation and as an ovipositor, or egg-laying organ. About 98% of moth species have a separate organ for mating, and an external duct that carries the sperm from the male.^[23]:561

The abdomen of the caterpillar has four pairs of prolegs, normally located on the third to sixth segments of the abdomen, and a separate pair of prolegs by the anus, which have a pair of tiny hooks called crotchets. These aid in gripping and walking, especially in species that lack many prolegs (e. g. larvae of Geometridae). In some basal moths, these prolegs may be on every segment of the body, while prolegs may be completely absent in other groups, which are more adapted to boring and living in sand (e. g., Prodoxidae and Nepticulidae, respectively).^[23]:563

Scales

Wing scales form the color and pattern on wings. The scales shown here are lamellar. The pedicel can be seen attached to a few loose scales.

The wings, head, and parts of the thorax and abdomen of Lepidoptera are covered with minute scales, a feature from which the order derives its name. Most scales are lamellar, or blade-like and attached with a pedicel, while other forms may be hair-like or specialized as secondary sexual characteristics.^[27]

The lumen or surface of the lamella has a complex structure. It gives color either by colored pigments it contains, or through structural coloration with mechanisms that include photonic crystals and diffraction gratings.^[28]

Scales function in insulation, thermoregulation, producing pheromones (in males only),^[29] and aiding gliding flight, but the most important is the large diversity of vivid or indistinct patterns they provide, which help the organism protect itself by camouflage or mimicry, and which act as signals to other animals including rivals and potential mates.^[27]

Electron microscopy images of scales

A patch of wing (×50)

Scales close up (×200)

A single scale (×1000)

Microstructure of a scale (×5000)

Internal morphology

Reproductive system

In the reproductive system of butterflies and moths, the male genitalia are complex and unclear. In females the three types of genitalia are based on the relating taxa: 'monotrysian', 'exoporian', and 'ditrysian'. In the monotrysian type is an opening on the fused segments of the sterna 9 and 10, which act as insemination and oviposition. In the exoporian type (in Hepaloidae and Mnesarchaeoidea) are two separate places for insemination and oviposition, both occurring on the same sterna as the monotrysian type, i.e. 9 and 10.^[21] The ditrysian groups have an internal duct that carries sperm, with separate openings for copulation and egg-laying.^[4] In most species, the genitalia are flanked by two soft lobes, although they may be specialized and sclerotized in some species for ovipositing in area such as crevices and inside plant tissue.^[21] Hormones and the glands that produce them run the development of butterflies and moths as they go through their life cycles, called the endocrine system. The first insect hormone prothoracicotropic hormone (PTTH) operates the species life cycle and diapause.^[30] This hormone is produced by corpora allata and corpora cardiaca, where it is also stored. Some glands are specialized to perform certain task such as producing silk or producing saliva in the palpi.^{[31]:65, 75} While the corpora cardiaca produce PTTH, the corpora allata also produces juvenile hormones, and the prothorocic glands produce moulting hormones.

Digestive system

In the digestive system, the anterior region of the foregut has been modified to form a pharyngeal sucking pump as they need it for the food they eat, which are for the most part liquids. An esophagus follows and leads to the posterior of the pharynx and in some species forms a form of crop. The midgut is short and straight, with the hindgut being longer and coiled.^[21] Ancestors of lepidopteran species, stemming from Hymenoptera, had midgut ceca, although this is lost in current butterflies and moths. Instead, all the digestive enzymes, other than initial digestion, are immobilized at the surface of the midgut cells. In larvae, long-necked and stalked goblet cells are found in the anterior and posterior midgut regions, respectively. In insects, the goblet cells excrete positive potassium ions, which are absorbed from leaves ingested by the larvae. Most butterflies and moths display the usual digestive cycle, but species with different diets require adaptations to meet these new demands.^[23]:279

Internal morphology of adult male in the family Nymphalidae, showing most of the major organ systems, with characteristic reduced forelegs of that family: The corpora include the corpus allatum and the corpus cardiaca.

Circulatory system

In the circulatory system, hemolymph, or insect blood, is used to circulate heat in a form of thermoregulation, where muscles contraction produces heat, which is transferred to the rest of the body when conditions are unfavorable.^[32]
In lepidopteran species, hemolymph is circulated through the veins in the wings by some form of pulsating organ, either by the heart or by the intake of air into the trachea.^[31]:69

Respiratory system

Air is taken in through spiracles along the sides of the abdomen and thorax supplying the trachea with oxygen as it goes through the lepidopteran's respiratory system. Three different tracheaes supply and diffuse oxygen throughout the species' bodies. The dorsal tracheae supply oxygen to the dorsal musculature and vessels, while the ventral tracheae supply the ventral musculature and nerve cord, and the visceral tracheae supply the guts, fat bodies, and gonads.^{[31]:71, 72}

Polymorphism

Sexually dimorphic bagworm moths (Thyridopteryx ephemeraeformis) mating: The female is flightless.

The Heliconius butterflies from the tropics of the Western Hemisphere are the classical model for Müllerian mimicry.

Polymorphism is the appearance of forms or "morphs", which differ in color and number of attributes within a single species.^[12]:163[33] In Lepidoptera, polymorphism can be seen not only between individuals in a population, but also between the sexes as sexual dimorphism, between geographically separated populations in geographical polymorphism, and between generations flying at different seasons of the year (seasonal polymorphism or polyphenism). In some species, the polymorphism is limited to one sex, typically the female. This often includes the phenomenon of mimicry when mimetic morphs fly alongside nonmimetic morphs in a population of a particular species. Polymorphism occurs both at specific level with heritable variation in the overall morphological adaptations of individuals, as well as in certain specific morphological or physiological traits within a species.^[12]

Environmental polymorphism, in which traits are not inherited, is often termed as polyphenism, which in Lepidoptera is commonly seen in the form of seasonal morphs, especially in the butterfly families of Nymphalidae and Pieridae. An Old World pierid butterfly, the common grass yellow (Eurema hecabe) has a darker summer adult morph, triggered by a long day exceeding 13 hours in duration, while the shorter diurnal period of 12 hours or less induces a paler morph in the postmonsoon period.^[34] Polyphenism also occurs in caterpillars, an example being the peppered moth, Biston betularia.^[35]

Geographical isolation causes a divergence of a species into different morphs. A good example is the Indian white admiral Limenitis procris, which has five forms, each geographically separated from the other by large mountain ranges.^[36]:26 An even more dramatic showcase of geographical polymorphism is the Apollo butterfly (Parnassius apollo). Because the Apollos live in small local populations, thus having no contact with each other, coupled with their strong stenotopic nature and weak migration ability, interbreeding between populations of one species practically does not occur; by this, they form over 600 different morphs, with the size of spots on the wings of which varies greatly.^[37]

Seasonal diphenism in the common grass yellow, Eurema hecabe

Dry-season form

Wet-season form

Sexual dimorphism is the occurrence of differences between males and females in a species. In Lepidoptera, it is widespread and almost completely set by genetic determination.^[34] Sexual dimorphism is present in all families of the Papilionoidea and more prominent in the Lycaenidae, Pieridae, and certain taxa of the Nymphalidae. Apart from color variation, which may differ from slight to completely different color-pattern combinations, secondary sexual characteristics may also be present.^[36]:25 Different genotypes maintained by natural selection may also be expressed at the same time.^[34] Polymorphic and/or mimetic females occur in the case of some taxa in the Papilionidae primarily to obtain a level of protection not available to the male of their species. The most distinct case of sexual dimorphism is that of adult females of many Psychidae species which have only vestigial wings, legs, and mouthparts as compared to the adult males that are strong fliers with well-developed wings and feathery antennae.^[38]

Reproduction and development

Mating pair of Laothoe populi (poplar hawk-moth) showing two different color variants

Species of Lepidoptera undergo holometabolism or "complete metamorphosis". Their life cycle normally consists of an egg, a larva, a pupa, and an imago or adult.^[12] The larvae are commonly called caterpillars, and the pupae of moths encapsulated in silk are called cocoons, while the uncovered pupae of butterflies are called chrysalides.

Lepidopterans in diapause

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Unless the species reproduces year-round, a butterfly or moth may enter diapause, a state of dormancy that allows the insect to survive unfavorable environmental conditions.

Mating

Males usually start eclosion (emergence) earlier than females and peak in numbers before females. Both of the sexes are sexually mature by the time of eclosion.^[23]:564 Butterflies and moths normally do not associate with each other, except for migrating species, staying relatively asocial. Mating begins with an adult (female or male) attracting a mate, normally using visual stimuli, especially in diurnal species like most butterflies. However, the females of most nocturnal species, including almost all moth species, use pheromones to attract males, sometimes from long distances.^[12] Some species engage in a form of acoustic courtship, or attract mates using sound or vibration such as the polka-dot wasp moth, Syntomeida epilais.^[39]

Adaptations include undergoing one seasonal generation, two or even more, called voltinism (Univoltism, bivoltism, and multivism, respectively). Most lepidopterans in temperate climates are univoltine, while in tropical climates most have two seasonal broods. Some others may take advantage of any opportunity they can get, and mate continuously throughout the year. These seasonal adaptations are controlled by hormones, and these delays in reproduction are called diapause.^[23]:567 Many lepidopteran species, after mating and laying their eggs, die shortly afterwards, having only lived for a few days after eclosion. Others may still be active for several weeks and then overwinter and become sexually active again when the weather becomes more favorable, or diapause. The sperm of the male that mated most recently with the female is most likely to have fertilized the eggs, but the sperm from a prior mating may still prevail.^[23]:564

Life cycle

The four stages of the life cycle of an anise swallowtail

Eggs

Lepidoptera usually reproduce sexually and are oviparous (egg-laying), though some species exhibit live birth in a process called ovoviviparity. A variety of differences in egg-laying and the number of eggs laid occur. Some species simply drop their eggs in flight (these species normally have polyphagous larvae, meaning they eat a variety of plants e. g., hepialids and some nymphalids)^[40] while most lay their eggs near or on the host plant on which the larvae feed. The number of eggs laid may vary from only a few to several thousand.^[12] The females of both butterflies and moths select the host plant instinctively, and primarily, by chemical cues.^[23]:564

The eggs are derived from materials ingested as a larva and in some species, from the spermatophores received from males during mating.^[41] An egg can only be 1/1000 the mass of the female, yet she may lay up to her own mass in eggs. Females lay smaller eggs as they age. Larger females lay larger eggs.^[42] The egg is covered by a hard-ridged protective outer layer of shell, called the chorion. It is lined with a thin coating of wax, which prevents the egg from drying out. Each egg contains a number of micropyles, or tiny funnel-shaped openings at one end, the purpose of which is to allow sperm to enter and fertilize the egg. Butterfly and moth eggs vary greatly in size between species, but they are all either spherical or ovate.

The egg stage lasts a few weeks in most butterflies, but eggs laid prior to winter, especially in temperate regions, go through diapause, and hatching may be delayed until spring. Other butterflies may lay their eggs in the spring and have them hatch in the summer. These butterflies are usually temperate species (e. g. Nymphalis antiopa).

Larvae

Larval form typically lives and feeds on plants

The larvae or caterpillars are the first stage in the life cycle after hatching. Caterpillars, are "characteristic polypod larvae with cylindrical bodies, short thoracic legs, and abdominal prolegs (pseudopods)".^[43] They have a toughened (sclerotised) head capsule with an adfrontal suture formed by medial fusion of the sclerites, mandibles (mouthparts) for chewing, and a soft tubular, segmented body, that may have hair-like or other projections, three pairs of true legs, and additional prolegs (up to five pairs).^[44] The body consists of thirteen segments, of which three are thoracic and ten are abdominal.^[45] Most larvae are herbivores, but a few are carnivores (some eat ants or other caterpillars) and detritivores.^[44]

Different herbivorous species have adapted to feed on every part of the plant and are normally considered pests to their host plants; some species have been found to lay their eggs on the fruit and other species lay their eggs on clothing or fur (e. g., Tineola bisselliella, the common clothes moth). Some species are carnivorous and others are even parasitic. Some lycaenid species such as Maculinea rebeli are social parasites of Myrmica ants nests.^[46] A species of Geometridae from Hawaii has carnivorous larvae that catch and eat flies.^[47] Some pyralid caterpillars are aquatic.^[48]

The larvae develop rapidly with several generations in a year; however, some species may take up to 3 years to develop, and exceptional examples like Gynaephora groenlandica take as long as seven years.^[12] The larval stage is where the feeding and growing stages occur, and the larvae periodically undergo hormone-induced ecdysis, developing further with each instar, until they undergo the final larval-pupal molt.

The larvae of both butterflies and moths exhibit mimicry to deter potential predators. Some caterpillars have the ability to inflate parts of their heads to appear snake-like. Many have false eye-spots to enhance this effect. Some caterpillars have special structures called osmeteria (family Papilionidae), which are exposed to produce smelly chemicals used in defense. Host plants often have toxic substances in them and caterpillars are able to sequester these substances and retain them into the adult stage. This helps make them unpalatable to birds and other predators. Such unpalatability is advertised using bright red, orange, black, or white warning colors. The toxic chemicals in plants are often evolved specifically to prevent them from being eaten by insects. Insects, in turn, develop countermeasures or make use of these toxins for their own survival. This "arms race" has led to the coevolution of insects and their host plants.^[49]

Wing development

No form of wing is externally visible on the larva, but when larvae are dissected, developing wings can be seen as disks, which can be found on the second and third thoracic segments, in place of the spiracles that are apparent on abdominal segments. Wing disks develop in association with a trachea that runs along the base of the wing, and are surrounded by a thin peripodial membrane, which is linked to the outer epidermis of the larva by a tiny duct. Wing disks are very small until the last larval instar, when they increase dramatically in size, are invaded by branching tracheae from the wing base that precede the formation of the wing veins, and begin to develop patterns associated with several landmarks of the wing.^[50]

Near pupation, the wings are forced outside the epidermis under pressure from the hemolymph, and although they are initially quite flexible and fragile, by the time the pupa breaks free of the larval cuticle, they have adhered tightly to the outer cuticle of the pupa (in obtect pupae). Within hours, the wings form a cuticle so hard and well-joined to the body that pupae can be picked up and handled without damage to the wings.^[50]

Pupa

Play media

Eclosion of Papilio dardanus

After about five to seven instars,^[51]:26–28 or molts, certain hormones, like PTTH, stimulate the production of ecdysone, which initiates insect molting. Then, the larva puparium, a sclerotized or hardened cuticle of the last larval instar, develops into the pupa. Depending on the species, the pupa may be covered in a silk cocoon, attached to different types of substrates, buried in the ground, or may not be covered at all. Features of the imago are externally recognizable in the pupa. All the appendages on the adult head and thorax are found cased inside the cuticle (antennae, mouthparts, etc.), with the wings wrapped around, adjacent to the antennae.^[23]:564 The pupae of some species have functional mandibles, while the pupal mandibles are not functional in others.^[22]

While encased, some of the lower segments are not fused, and are able to move using small muscles found in between the membrane. Moving may help the pupa, for example, escape the sun, which would otherwise kill it. The pupa of the Mexican jumping bean moth (Cydia deshaisiana) does this. The larvae cut a trapdoor in the bean (species of Sebastiania) and use the bean as a shelter. With a sudden rise in temperature, the pupa inside twitches and jerks, pulling on the threads inside. Wiggling may also help to deter parasitoid wasps from laying eggs on the pupa. Other species of moths are able to make clicks to deter predators.^{[23]:564, 566}

The length of time before the pupa ecloses (emerges) varies greatly. The monarch butterfly may stay in its chrysalis for two weeks, while other species may need to stay for more than 10 months in diapause. The adult emerges from the pupa either by using abdominal hooks or from projections located on the head. The mandibles found in the most primitive moth families are used to escape from their cocoon (e. g., Micropterigoidea).^[12][23]:564

Adult

Most lepidopteran species do not live long after eclosion, only needing a few days to find a mate and then lay their eggs. Others may remain active for a longer period (from one to several weeks), or go through diapause and overwintering as monarch butterflies do, or waiting out environmental stress. Some adult species of microlepidoptera go through a stage where no reproductive-related activity occurs, lasting through summer and winter, followed by mating and oviposition in the early spring.^[23]:564

While most butterflies and moths are terrestrial, many species of Pyralidae are truly aquatic with all stages except the adult occurring in water. Many species from other families such as Arctiidae, Nepticulidae, Cosmopterygidae, Tortricidae, Olethreutidae, Noctuidae, Cossidae, and Sphingidae are aquatic or semiaquatic.^[52]:22

Behavior

Flight

Flight is an important aspect of the lives of butterflies and moths, and is used for evading predators, searching for food, and finding mates in a timely manner, as lepidopteran species do not live long after eclosion. It is the main form of locomotion in most species. In Lepidoptera, the forewings and hindwings are mechanically coupled and flap in synchrony. Flight is anteromotoric, or being driven primarily by action of the forewings. Although lepidopteran species reportedly can still fly when their hindwings are cut off, it reduces their linear flight and turning capabilities.^[53]

Lepidopteran species have to be warm, about 77 to 79 °F (25 to 26 °C), to fly. They depend on their body temperature being sufficiently high and since they cannot regulate it themselves, this is dependent on their environment. Butterflies living in cooler climates may use their wings to warm their bodies. They will bask in the sun, spreading out their wings so that they get maximum exposure to the sunlight. In hotter climates butterflies can easily overheat, so they are usually active only during the cooler parts of the day, early morning, late afternoon or early evening. During the heat of the day, they rest in the shade. Some larger thick-bodied moths (e.g. Sphingidae) can generate their own heat to a limited degree by vibrating their wings. The heat generated by the flight muscles warms the thorax while the temperature of the abdomen is unimportant for flight. To avoid overheating, some moths rely on hairy scales, internal air sacs, and other structures to separate the thorax and abdomen and keep the abdomen cooler.^[54]

Some species of butterflies can reach fast speeds, such as the southern dart, which can go as fast as 48.4 km/h. Sphingids are some of the fastest flying insects, some are capable of flying at over 50 km/h (30 mi/h), having a wingspan of 35–150 mm.^[1][55] In some species, sometimes a gliding component to their flight exists. Flight occurs either as hovering, or as forward or backward motion.^[56] In butterfly and moth species, such as hawk moths, hovering is important as they need to maintain a certain stability over flowers when feeding on the nectar.^[1]

Navigation

Timelapse of flying moths, attracted to the floodlights

Navigation is important to Lepidoptera species, especially for those that migrate. Butterflies, which have more species that migrate, have been shown to navigate using time-compensated sun compasses. They can see polarized light, so can orient even in cloudy conditions. The polarized light in the region close to the ultraviolet spectrum is suggested to be particularly important.^[57] Most migratory butterflies are those that live in semiarid areas where breeding seasons are short.^[58] The life histories of their host plants also influence the strategies of the butterflies.^[59] Other theories include the use of landscapes. Lepidoptera may use coastal lines, mountains, and even roads to orient themselves. Above sea, the flight direction is much more accurate if the coast is still visible.^[60]

Many studies have also shown that moths navigate. One study showed that many moths may use the Earth's magnetic field to navigate, as a study of the moth heart and dart suggests.^[61] Another study, of the migratory behavior of the silver Y, showed, even at high altitudes, the species can correct its course with changing winds, and prefers flying with favourable winds, suggesting a great sense of direction.^[62][63]Aphrissa statira in Panama loses its navigational capacity when exposed to a magnetic field, suggesting it uses the Earth's magnetic field.^[64]

Moths exhibit a tendency to circle artificial lights repeatedly. This suggests they use a technique of celestial navigation called transverse orientation. By maintaining a constant angular relationship to a bright celestial light, such as the Moon, they can fly in a straight line. Celestial objects are so far away, even after traveling great distances, the change in angle between the moth and the light source is negligible; further, the moon will always be in the upper part of the visual field or on the horizon. When a moth encounters a much closer artificial light and uses it for navigation, the angle changes noticeably after only a short distance, in addition to being often below the horizon. The moth instinctively attempts to correct by turning toward the light, causing airborne moths to come plummeting downwards, and at close range, which results in a spiral flight path that gets closer and closer to the light source.^[65] Other explanations have been suggested, such as the idea that moths may be impaired with a visual distortion called a Mach band by Henry Hsiao in 1972. He stated that they fly towards the darkest part of the sky in pursuit of safety, thus are inclined to circle ambient objects in the Mach band region.^[66]

Migration

Monarch butterflies, seen in a cluster in Santa Cruz, California, where the western population migrates for the winter

Lepidopteran migration is typically seasonal, as the insects moving to escape dry seasons or other disadvantageous conditions. Most lepidopterans that migrate are butterflies, and the distance travelled varies. Some butterflies that migrate include the mourning cloak, painted lady, American lady, red admiral, and the common buckeye.^[51]:29–30 A notable species of moth that migrates long distances is the bogong moth.^[67] The most well-known migrations are those of the eastern population of the monarch butterfly from Mexico to northern United States and southern Canada, a distance of about 4,000–4,800 km (2,500–3,000 mi). Other well-known migratory species include the painted lady and several of the danaine butterflies. Spectacular and large-scale migrations associated with the monsoons are seen in peninsular India.^[68] Migrations have been studied in more recent times using wing tags and stable hydrogen isotopes.^[69][70]

Moths also undertake migrations, an example being the uraniids. Urania fulgens undergoes population explosions and massive migrations that may be not surpassed by any other insect in the Neotropics. In Costa Rica and Panama, the first population movements may begin in July and early August and depending on the year, may be very massive, continuing unabated for as long as five months.^[71]

Communication

Group of Melitaea athalia near Warka, Poland

Pheromones are commonly involved in mating rituals among species, especially moths, but they are also an important aspect of other forms of communication. Usually, the pheromones are produced by either the male or the female and detected by members of the opposite sex with their antennae.^[72] In many species, a gland between the eighth and ninth segments under the abdomen in the female produces the pheromones.^[12] Communication can also occur through stridulation, or producing sounds by rubbing various parts of the body together.^[63]

Moths are known to engage in acoustic forms of communication, most often as courtship, attracting mates using sound or vibration. Like most other insects, moths pick up these sounds using tympanic membranes in their abdomens.^[73] An example is that of the polka-dot wasp moth (Syntomeida epilais), which produces sounds with a frequency above that normally detectable by humans (about 20 kHz). These sounds also function as tactile communication, or communication through touch, as they stridulate, or vibrate a substrate like leaves and stems.^[39]

Most moths lack bright colors, as many species use coloration as camouflage, but butterflies engage in visual communication. Female cabbage butterflies, for example, use ultraviolet light to communicate, with scales colored in this range on the dorsal wing surface. When they fly, each down stroke of the wing creates a brief flash of ultraviolet light which the males apparently recognize as the flight signature of a potential mate. These flashes from the wings may attract several males that engage in aerial courtship displays.^[73]

Ecology

Moths and butterflies are important in the natural ecosystem. They are integral participants in the food chain; having co-evolved with flowering plants and predators, lepidopteran species have formed a network of trophic relationships between autotrophs and heterotrophs, which are included in the stages of Lepidoptera larvae, pupae, and adults. Larvae and pupae are links in the diets of birds and parasitic entomophagous insects. The adults are included in food webs in a much broader range of consumers (including birds, small mammals, reptiles, etc.).^[23]:567

Defense and predation

Papilio machaon caterpillar showing the osmeterium, which emits unpleasant smells to ward off predators

Lepidopteran species are soft bodied, fragile, and almost defenseless, while the immature stages move slowly or are immobile, hence all stages are exposed to predation. Adult butterflies and moths are preyed upon by birds, bats, lizards, amphibians, dragonflies, and spiders. Caterpillars and pupae fall prey not only to birds, but also to invertebrate predators and small mammals, as well as fungi and bacteria. Parasitoid and parasitic wasps and flies may lay eggs in the caterpillar, which eventually kill it as they hatch inside its body and eat its tissues. Insect-eating birds are probably the largest predators. Lepidoptera, especially the immature stages, are an ecologically important food to many insectivorous birds, such as the great tit in Europe.

An "evolutionary arms race" can be seen between predator and prey species. The Lepidoptera have developed a number of strategies for defense and protection, including evolution of morphological characters and changes in ecological lifestyles and behaviors. These include aposematism, mimicry, camouflage, and development of threat patterns and displays.^[74] Only a few birds, such as the nightjars, hunt nocturnal lepidopterans. Their main predators are bats. Again, an "evolutionary race" exists, which has led to numerous evolutionary adaptations of moths to escape from their main predators, such as the ability to hear ultrasonic sounds, or even to emit sounds in some cases. Lepidopteran eggs are also preyed upon. Some caterpillars, such as the zebra swallowtail butterfly larvae, are cannibalistic.

Some species of Lepidoptera are poisonous to predators, such as the monarch butterfly in the Americas, Atrophaneura species (roses, windmills, etc.) in Asia, as well as Papilio antimachus, and the birdwings, the largest butterflies in Africa and Asia, respectively. They obtain their toxicity by sequestering the chemicals from the plants they eat into their own tissues. Some Lepidoptera manufacture their own toxins. Predators that eat poisonous butterflies and moths may become sick and vomit violently, learning not to eat those species. A predator which has previously eaten a poisonous lepidopteran may avoid other species with similar markings in the future, thus saving many other species, as well.^[74][75] Toxic butterflies and larvae tend to develop bright colors and striking patterns as an indicator to predators about their toxicity. This phenomenon is known as aposematism.^[76] Some caterpillars, especially members of Papilionidae, contain an osmeterium, a Y-shaped protrusible gland found in the prothoracic segment of the larvae.^[74] When threatened, the caterpillar emits unpleasant smells from the organ to ward off the predators.^[77][78]

Camouflage is also an important defense strategy, which involves the use of coloration or shape to blend into the surrounding environment. Some lepidopteran species blend with their surroundings, making them difficult to spot by predators. Caterpillars can exhibit shades of green that match its host plant. Others look like inedible objects, such as twigs or leaves. For instance, the mourning cloak fades into the backdrop of trees when it folds its wings back. The larvae of some species, such as the common Mormon (Papilio polytes) and the western tiger swallowtail look like bird droppings.^[74][79] For example, adult Sesiidae species (also known as clearwing moths) have a general appearance sufficiently similar to a wasp or hornet to make it likely the moths gain a reduction in predation by Batesian mimicry.^[80]Eyespots are a type of automimicry used by some butterflies and moths. In butterflies, the spots are composed of concentric rings of scales in different colors. The proposed role of the eyespots is to deflect attention of predators. Their resemblance to eyes provokes the predator's instinct to attack these wing patterns.^[81]

Batesian and Müllerian mimicry complexes are commonly found in Lepidoptera. Genetic polymorphism and natural selection give rise to otherwise edible species (the mimic) gaining a survival advantage by resembling inedible species (the model). Such a mimicry complex is referred to as Batesian and is most commonly known in the example between the limenitidine viceroy butterfly in relation to the inedible danaine monarch. The viceroy is, in fact, more toxic than the monarch and this resemblance should be considered as a case of Müllerian mimicry.^[82] In Müllerian mimicry, inedible species, usually within a taxonomic order, find it advantageous to resemble each other so as to reduce the sampling rate by predators that need to learn about the insects' inedibility. Taxa from the toxic genus Heliconius form one of the most well-known Müllerian complexes.^[83] The adults of the various species now resemble each other so well, the species cannot be distinguished without close morphological observation and, in some cases, dissection or genetic analysis.

Moths evidently are able to hear the range emitted by bats, which in effect causes flying moths to make evasive maneuvers because bats are a main predator of moths. Ultrasonic frequencies trigger a reflex action in the noctuid moth that cause it to drop a few inches in its flight to evade attack.^[84]Tiger moths in a defense emit clicks within the same range of the bats, which interfere with the bats and foil their attempts to echolocate it.^[85]

Pollination

A day-flying hummingbird hawk-moth drinking nectar from a species of Dianthus

Most species of Lepidoptera engage in some form of entomophily (more specifically psychophily and phalaenophily for butterflies and moths, respectively), or the pollination of flowers.^[86] Most adult butterflies and moths feed on the nectar inside flowers, using their probosces to reach the nectar hidden at the base of the petals. In the process, the adults brush against the flowers' stamens, on which the reproductive pollen is made and stored. The pollen is transferred on appendages on the adults, which fly to the next flower to feed and unwittingly deposit the pollen on the stigma of the next flower, where the pollen germinates and fertilizes the seeds.^{[23]:813–814}

Flowers pollinated by butterflies tend to be large and flamboyant, pink or lavender in color, frequently having a landing area, and usually scented, as butterflies are typically day-flying. Since butterflies do not digest pollen (except for heliconid species,^[86]) more nectar is offered than pollen. The flowers have simple nectar guides, with the nectaries usually hidden in narrow tubes or spurs, reached by the long "tongue" of the butterflies. Butterflies such as Thymelicus flavus have been observed to engage in flower constancy, which means they are more likely to transfer pollen to other conspecific plants. This can be beneficial for the plants being pollinated, as flower constancy prevents the loss of pollen during different flights and the pollinators from clogging stigmas with pollen of other flower species.^[87]

Among the more important moth pollinator groups are the hawk moths of the family Sphingidae. Their behavior is similar to hummingbirds, i.e., using rapid wing beats to hover in front of flowers. Most hawk moths are nocturnal or crepuscular, so moth-pollinated flowers (e.g., Silene latifolia ) tend to be white, night-opening, large, and showy with tubular corollae and a strong, sweet scent produced in the evening, night, or early morning. A lot of nectar is produced to fuel the high metabolic rates needed to power their flight.^[88] Other moths (e.g., noctuids, geometrids, pyralids) fly slowly and settle on the flower. They do not require as much nectar as the fast-flying hawk moths, and the flowers tend to be small (though they may be aggregated in heads).^[89]

Mutualism

Tobacco hornworm caterpillar (Manduca sexta) parasitized by Braconidae wasp larvae

Mutualism is a form of biological interaction wherein each individual involved benefits in some way. An example of a mutualistic relationship would be that shared by yucca moths (Tegeculidae) and their host, yucca flowers (Liliaceae). Female yucca moths enter the host flowers, collect the pollen into a ball using specialized maxillary palps, then move to the apex of the pistil, where pollen is deposited on the stigma, and lay eggs into the base of the pistil where seeds will develop. The larvae develop in the fruit pod and feed on a portion of the seeds. Thus, both insect and plant benefit, forming a highly mutualistic relationship.^[23]:814 Another form of mutualism occurs between some larvae of butterflies and certain species of ants (e. g. Lycaenidae). The larvae communicate with the ants using vibrations transmitted through a substrate, such as the wood of a tree or stems, as well as using chemical signals.^[90] The ants provide some degree of protection to these larvae and they in turn gather honeydew secretions.^[91]

Parasitism

Parasitoid larvae exits from the fox moth caterpillar

Only 42 species of parasitoid lepidopterans are known (1 Pyralidae; 40 Epipyropidae).^[23]:748 The larvae of the greater and lesser wax moths feed on the honeycomb inside bee nests and may become pests; they are also found in bumblebee and wasp nests, albeit to a lesser extent. In northern Europe, the wax moth is regarded as the most serious parasitoid of the bumblebee, and is found only in bumblebee nests. In some areas in southern England, as much as 80% of nests can be destroyed.^[92] Other parasitic larvae are known to prey upon cicadas and leaf hoppers.^[93]