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I am using VTK with Activiz.net to reconstruct a JPEG ultrasound series, I have used raycasting technique and applied transfer function as here,
The problem is that the Opacity transfer function isn't working with the vtkJPEG reader output, the black regions were not filtered, the code is working fine with vtkDICOMReader? so, any solutions for this ???

The code:

 vtkJPEGReader jpegReader = vtkJPEGReader.New();
 jpegReader.SetDataByteOrderToLittleEndian();
 jpegReader.SetFilePattern("%s%d.jpg");
 jpegReader.SetFilePrefix(@"C:UsersDolaDesktoptestTestC#SeriesFrame_001");
 jpegReader.SetDataExtent(0, Width, 0, Length,1, numberOfFrames);
 jpegReader.SetDataScalarTypeToFloat();
 jpegReader.SetDataSpacing(1, 1, 1);
 jpegReader.SetDataOrigin(0, 0, 0);



 vtkImageThreshold SelectTissue = vtkImageThreshold.New();
 SelectTissue.SetInput(jpegReader.GetOutput());
 SelectTissue.ThresholdBetween(0, 10);

 vtkFixedPointVolumeRayCastMapper volumeMapper = vtkFixedPointVolumeRayCastMapper.New();
 volumeMapper.SetInput(SelectTissue.GetOutput());

 vtkColorTransferFunction volumeColor = vtkColorTransferFunction.New();
 volumeColor.AddRGBPoint(1, 1, 0, 0);
 volumeColor.AddRGBPoint(100, 1, 0, 0.4);
 volumeColor.AddRGBPoint(160, 1.0, 0.5, 0.1);
 volumeColor.AddRGBPoint(255, 1.0, 0.5, 0.1);


 vtkPiecewiseFunction volumeScalarOpacity = vtkPiecewiseFunction.New();
 volumeScalarOpacity.AddPoint(0, 0.00);
 volumeScalarOpacity.AddPoint(20, 0.00);
 volumeScalarOpacity.AddPoint(30, 0.20);
 volumeScalarOpacity.AddPoint(100, 0.7);


 vtkPiecewiseFunction volumeGradientOpacity = vtkPiecewiseFunction.New();
 volumeGradientOpacity.AddPoint(0, 1.0);
 volumeGradientOpacity.AddPoint(255, 1.0);

 vtkVolumeProperty volumeProperty = vtkVolumeProperty.New();
 volumeProperty.SetColor(volumeColor);
 volumeProperty.SetScalarOpacity(volumeScalarOpacity);
 volumeProperty.SetGradientOpacity(volumeGradientOpacity);
 volumeProperty.SetInterpolationTypeToLinear();
 volumeProperty.ShadeOn();
 volumeProperty.SetAmbient(0.1);
 volumeProperty.SetDiffuse(0.9);
 volumeProperty.SetSpecular(0.2);

 vtkVolume volume = vtkVolume.New();
 volume.SetMapper(volumeMapper);
 volume.SetProperty(volumeProperty);


 vtkRenderWindow renderWindow = renderWindowControl1.RenderWindow;
 vtkRenderer renderer = renderWindow.GetRenderers().GetFirstRenderer();
 renderer.AddViewProp(volume);
 renderer.SetBackground(0.2, 0.3, 0.4);